|Dario Simões Zamboni
Tel.: (16) 3315-3265
The Laboratory of Innate Immunity and Microbial Pathogenesis (Zamboni Lab) was established in 2006, when Dario S. Zamboni was hired at the Medical School of Ribeirão Preto, University of São Paulo (FMRP/USP).
The Lab investigates the interactions of intracellular microbial pathogens with host cells, a multidisciplinary area of research involving Immunology, Cell Biology and Microbiology. The pathogens under investigation include the intracellular parasites Leishmania and Trypanosoma cruzi, and the intracellular bacteria Legionella and Coxiella burnetii.
We investigate how the immune system detects microbial pathogens and operate to clear infection. We also investigate how the intracellular pathogens subvert the host cell functions to replicate intracellularly and cause disease. To achieve these goals, we use modern tools of molecular biology, biochemistry, and genetics.
The Laboratory is located at the Department of Cellular and Molecular Biology of the FMRP/USP and is funded FAPESP, CNPq, CAPES, WHO, FAEPA and PEW. We are part of the Center of Research in Inflammatory Diseases (CRID/FAPESP) and the National Institute for Science, Technology and Vaccines (INCTV/CNPq).
The following research lines are being conducted in the laboratory:
1) Recognition of intracellular pathogens by cytoplasmic pattern recognition receptors and their importance in host resistance
(Reconhecimento de patógenos intracelulares por receptores citoplasmáticos e sua importância no controle da infecção microbiana)
2) Molecular pathogenesis and subversion of host responses in infections by intracellular pathogens
(Patogênese molecular e subversão das respostas do hospedeiro em infecções por patógenos intracelulares)
3) Determination of mammalian genes and loci responsible for resistance against an infection by intracellular pathogens
(Determinação de genes e loci de mamíferos responsáveis pela resistência contra a infecção por patógenos intracelulares)
4) Use of high-content screening to identify compounds with leishmanicidal activity in Leishmania-infected macrophages
(Utilização de varredura por high-content para identificar compostos com atividade leishmanicida em macrófagos infectados por Leishmania)
5) Development of genome-wide, high-throughput screening (using CRISPR/CAS9) to identify novel pathways involved in host response to intracellular pathogens.
(Desenvolvimento de genome-wide, high-throughput screening (por CRISPR/CAS9) para identificar novas vias de sinalização envolvidas na resposta do hospedeiro contra patógenos intracelulares)